国家水生生物种质资源库国家斑马鱼资源中心 (China Zebrafish Resource Center)

ZKO number		ZKO773
Recent progress of this line		F1 individuals have been identified
Gene name		hmx1
Ensembl ID		ENSDARG00000095651
Zebrafish strain		AB
Cas9 target site 1 sequence		sense strand CCATTATTCAGGTCCAAGAGagg
Cas9 target site 2 sequence
Position in the gene		intron1-2 and exon2
Restriction enzyme (RE)		n/a
PCR primer (forward)		TGAAAGCCACAGGGAGA
PCR primer (reverse)		GAGCCGGGTTCGGAAGT
Length of expected PCR product (bp)		369
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA		pXT7-hCAS9
Plasmids?used?for?making?gRNA		pMD19T-gRNA
Primers?used?for?making?gRNA		Pr-ZKO 773
Dosage of injected Cas9 mRNA (ng/ul)		150
Dosage of injected gRNA (ng/ul)		100
Survival rate of F0 embryo @ 1dpf (%)
Method to estimate mutation efficiency		sequencing of PCR product
Stage of the embryos collected for mutation detection		http://www.zfish.cn/upload/file/ZKOPDF/ZKO773.pdf
Mutation efficiency in F0 embryos (%)
Picture?from?F₀?embryos ? (gel?picture?or?sequencing?picture)		/upload/2014-11-28/635527853721721166.png 图片2
Mutation type		Indel
Germline transmission efficiency in adult F₀
Wild-type (WT) sequence
Germline mutations in selected F0 (By screening F1 embryos)	F0-1
Germline mutations in selected F0 (By screening F1 embryos)	F0-2
Mosaicism in selected F0 (By screening F1 adults)	F0-1
Mosaicism in selected F0 (By screening F1 adults)	F0-2
Allele name and predicted product	773a 773b
Allele name and predicted product	773a 773b
突变创建时间		2013-08-06
填表时间		2013/12/16
修改时间		2016/9/28
PI/负责人 (工作单位)

Genotype Information: