国家水生生物种质资源库国家斑马鱼资源中心 (China Zebrafish Resource Center)

ZKO number		ZKO785
Recent progress of this line		F1 individuals have been identified
Gene name		null
Ensembl ID		ENSDARG00000090867
Zebrafish strain		AB
Cas9 target site 1 sequence		antisense strand GGATACTGGTGAAGTGGATGtgg
Cas9 target site 2 sequence
Position in the gene		exon4
Restriction enzyme (RE)		BtsCI
PCR primer (forward)		5' ACCAGCATCAACAACTAA 3'
PCR primer (reverse)		5' TTGGCAATTTGGACAT 3'
Length of expected PCR product (bp)		389
Length of expected RE digestion product (bp)		274
Plasmids used for making Cas9 mRNA		pXT7-hCAS9
Plasmids?used?for?making?gRNA		pMD19T-gRNA
Primers?used?for?making?gRNA		Pr-ZKO 785
Dosage of injected Cas9 mRNA (ng/ul)		100
Dosage of injected gRNA (ng/ul)		200
Survival rate of F0 embryo @ 1dpf (%)		80
Method to estimate mutation efficiency		sequencing of PCR product
Stage of the embryos collected for mutation detection		http://www.zfish.cn/upload/file/ZKOPDF/ZKO785.pdf
Mutation efficiency in F0 embryos (%)		5
Picture?from?F₀?embryos ? (gel?picture?or?sequencing?picture)
Mutation type		Indel
Germline transmission efficiency in adult F₀		25
Wild-type (WT) sequence		TCACAACATCAACCTATGATGCCACATCCACTTCACCAGTATCCTCAATCATGGAATCAACATCA
Germline mutations in selected F0 (By screening F1 embryos)	F0-1
Germline mutations in selected F0 (By screening F1 embryos)	F0-2
Mosaicism in selected F0 (By screening F1 adults)	F0-1	TCACAACATCAACCTATGATGCCACATCCAGTATCCTCAATCATGGAATCAACATCAACA
Mosaicism in selected F0 (By screening F1 adults)	F0-2
Allele name and predicted product	785a 785b	TCACAACATCAACCTATGATGCCACATCCA-------GTATCCTCAATCATGGAATCAACATCAACA truncated protein 385aa
Allele name and predicted product	785a 785b
突变创建时间		2014-01-30
填表时间		2013/12/17
修改时间		2016/9/28
PI/负责人 (工作单位)

Genotype Information: