Chinese | English
用户名 密码 验证码 验证码
关闭
在线咨询
联系QQ:2178199170
如有订购ZKO项目创建品系需求,请点击ZKO品系购买进行下单,如有其它问题请参见ZKO订购说明。

ZKO number ZKO779
Recent progress of this line F2 generation has been raised
Gene name htt
Ensembl ID ENSDARG00000091911
Zebrafish strain AB
Cas9 target site 1 sequence antisense strand
GTTCCGTGTGCTTGCCCGCAtgg
Cas9 target site 2 sequence
Position in the gene exon 4
Restriction enzyme (RE) n/a
PCR primer (forward) TGCATTACTACACCTTTTCC
PCR primer (reverse) TCAGTCTTTCCCATGAGCCT
Length of expected PCR product (bp) 560
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids?used?for?making?gRNA pMD19T-gRNA
Primers?used?for?making?gRNA Pr-ZKO 779
Dosage of injected Cas9 mRNA (ng/ul) 150
Dosage of injected gRNA (ng/ul) 100
Survival rate of F0 embryo @ 1dpf (%)
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO779.pdf
Mutation efficiency in F0 embryos (%)
Picture?from?F0?embryos
? (gel?picture?or?sequencing?picture)
Mutation type deletion
Germline transmission efficiency in adult F0 25%
Wild-type (WT) sequence GTTCCGTGTGCTTGCCCGCATGGTGGACACTCTGGCCTGCAGACGT
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 -16: GTTCCGTGT----------------GACACTCTGGCCTGCAGACGT
F0-2 -4: GTTCCGTGTGCTT----GCATGGTGGACACTCTG
Allele name and predicted product 779a
779b
GTTCCGTGT----------------GACACTCTGGCCTGCAGACGT Shortened antisense RNA (886bp)
GTTCCGTGTGCTT----GCATGGTGGACACTCTG Shortened antisense RNA (898bp)
突变创建时间
填表时间 2013/12/18
修改时间 2016/9/28
PI/负责人 (工作单位)
Genotype Information:
友情链接: 更多
地址:武汉市东湖南路7号中科院水生所; 电话:027-68780570; 网址:http://www.zfish.cn/; 邮箱:[email protected]
Copyright © 2012 - 2023 国家斑马鱼资源中心 版权所有
鄂ICP备05003091号-2  鄂公网安备42010602003695