国家水生生物种质资源库国家斑马鱼资源中心 (China Zebrafish Resource Center)

ZKO number		ZKO810
Recent progress of this line		F2 individuals have been indentified
Gene name		scpp9
Ensembl ID		ENSDARG00000087178
Zebrafish strain		AB
Cas9 target site 1 sequence		sense strand tatgtgcttcagcctccagt
Cas9 target site 2 sequence
Position in the gene		exon5
Restriction enzyme (RE)		n/a
PCR primer (forward)		tcatcagggaagaaagcactat
PCR primer (reverse)		gattaggaagtccagccacg
Length of expected PCR product (bp)		549
Length of expected RE digestion product (bp)		348+201
Plasmids used for making Cas9 mRNA		pXT7-hCAS9
Plasmids?used?for?making?gRNA		pMD19T-gRNA
Primers?used?for?making?gRNA		Pr-ZKO 810
Dosage of injected Cas9 mRNA (ng/ul)		300
Dosage of injected gRNA (ng/ul)		20
Survival rate of F0 embryo @ 1dpf (%)		80
Method to estimate mutation efficiency		T7EI detection
Stage of the embryos collected for mutation detection		http://www.zfish.cn/upload/file/ZKOPDF/ZKO810.pdf
Mutation efficiency in F0 embryos (%)		50
Picture?from?F₀?embryos ? (gel?picture?or?sequencing?picture)		/upload/2014-09-04/635454438906560993.png zko810
Mutation type		Indel
Germline transmission efficiency in adult F₀		50%(3/3)
Wild-type (WT) sequence		GCTCTTCCTCCATATGTGC
Germline mutations in selected F0 (By screening F1 embryos)	F0-1
Germline mutations in selected F0 (By screening F1 embryos)	F0-2
Mosaicism in selected F0 (By screening F1 adults)	F0-1	GCTCTTCCTCTTCCTTCCTAAAACTATGTGC (+14 bp, -2 bp)
Mosaicism in selected F0 (By screening F1 adults)	F0-2
Allele name and predicted product	810a 810b	GCTCTTCCTCTTCCTTCCTAAAACTATGTGC Truncated protein (80aa)
Allele name and predicted product	810a 810b
突变创建时间
填表时间		2013/12/18
修改时间		2016/12/29
PI/负责人 (工作单位)

Genotype Information: